Engineers have used a new 3D bioprinting method to bioprint models of hearts, arteries, bones and brains out of biological materials
Bio

As of this month, over 4,000 Americans are on the waiting list to receive a heart transplant. With failing hearts, these patients have no other options; heart tissue, unlike other parts of the body, is unable to heal itself once it is damaged. Fortunately, recent work by a group at Carnegie Mellon could one day lead to a world in which transplants are no longer necessary to repair damaged organs.

“We’ve been able to take MRI images of coronary arteries and 3D images of embryonic hearts and 3D bioprint them with unprecedented resolution and quality out of very soft materials like collagens, alginates and fibrins,” said Adam Feinberg, an associate professor of Materials Science and Engineering and Biomedical Engineering at Carnegie Mellon University. Feinberg leads the Regenerative Biomaterials and Therapeutics Group, and the group’s study was published in the October 23 issue of the journal Science Advances. A demonstration of the technology can be viewed online.

“As excellently demonstrated by Professor Feinberg’s work in bioprinting, our CMU researchers continue to develop novel solutions like this for problems that can have a transformational effect on society,” said Jim Garrett, Dean of Carnegie Mellon’s College of Engineering. “We should expect to see 3D bioprinting continue to grow as an important tool for a large number of medical applications.”

Traditional 3-D printers build hard objects typically made of plastic or metal, and they work by depositing material onto a surface layer-by-layer to create the 3D object. Printing each layer requires sturdy support from the layers below, so printing with soft materials like gels has been limited.

“3D printing of various materials has been a common trend in tissue engineering in the last decade, but until now, no one had developed a method for assembling common tissue engineering gels like collagen or fibrin,” said TJ Hinton, a graduate student in biomedical engineering at Carnegie Mellon and lead author of the study.

“The challenge with soft materials – think about something like jello that we eat – is that they collapse under their own weight when 3D printed in air,” explained Feinberg. “So we developed a method of printing these soft materials inside a support bath material. Essentially, we print one gel inside of another gel, which allows us to accurately position the soft material as it’s being printed, layer-by-layer.”

One of the major advances of this technique, termed FRESH, or “Freeform Reversible Embedding of Suspended Hydrogels,” is that the support gel can be easily melted away and removed by heating to body temperature, which does not damage the delicate biological molecules or living cells that were bioprinted. As a next step, the group is working towards incorporating real heart cells into these 3D printed tissue structures, providing a scaffold to help form contractile muscle.

Bioprinting is a growing field, but to date, most 3D bioprinters have cost more than €100,000 and/or require specialised expertise to operate, limiting wider-spread adoption. Feinberg’s group, however, has been able to implement their technique on a range of consumer-level 3D printers, which cost less than €1,000 by utilising open-source hardware and software.

“Not only is the cost low, but by using open-source software, we have access to fine-tune the print parameters, optimise what we’re doing and maximise the quality of what we’re printing,” Feinberg said. “It has really enabled us to accelerate development of new materials and innovate in this space. And we are also contributing back by releasing our 3D printer designs under an open-source license.”

Journal Reference:

T. J. Hinton, Q. Jallerat, R. N. Palchesko, J. H. Park, M. S. Grodzicki, H.-J. Shue, M. H. Ramadan, A. R. Hudson, A. W. Feinberg. Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels. Science Advances, 2015; 1 (9): e1500758 DOI: 10.1126/sciadv.1500758

http://www.engineersjournal.ie/wp-content/uploads/2015/10/3-D-Printing.jpghttp://www.engineersjournal.ie/wp-content/uploads/2015/10/3-D-Printing-300x300.jpgDavid O'RiordanBio3D Printing,biomedical
As of this month, over 4,000 Americans are on the waiting list to receive a heart transplant. With failing hearts, these patients have no other options; heart tissue, unlike other parts of the body, is unable to heal itself once it is damaged. Fortunately, recent work by a group...